mouse anti sfrp2 antibody  (Proteintech)

Journal: Journal of Translational Autoimmunity

Article Title: TGF-β1 promotes collagen synthesis in systemic sclerosis via upregulating P4HA3

doi: 10.1016/j.jtauto.2025.100323

Figure Lengend Snippet: SFRP2 high fibroblasts showed the highest P4HA3 expression percentage. (A) Visualization clustering of 11,506 fibroblasts from 12 SSc patients and 10 healthy controls by UMAP plot. (B) Feature plots visualizing marker genes (SFRP2, CXCL12, TM4SF1, DPEP1, COCH), revealing distinct cell populations. (C) The expression level of P4HA3 in 5 subgroups of fibroblast subcluster. (D) The expression level of SFRP2 in 9 primary cell types. (E) Correlation between P4HA3 and SFRP2, CXCL12, TM4SF1, DPEP1 as well as COCH. ∗∗∗ P < 0.001 by Pearson correlation analysis. The data were downloaded from GSE181549 . (F) Visualization of top 5 GO enrichment results in biological process, cellular component and molecular function by enriching the upregulated differentially expressed genes in SSc fibroblasts between the SFRP2 high fibroblasts cluster and other 4 fibroblasts clusters. (G) Visualization of KEGG enrichment results using an adjusted p -value <0.05 as the threshold.

Article Snippet: Tissue slices were incubated overnight at 4 °C with rabbit anti-P4HA3 antibody (HPA007897, Sigma, 1:150) and mouse anti-SFRP2 antibody (66328-1-Ig, Proteintech, 1:100), followed by staining with CoraLite®555 conjugated goat anti-rabbit secondary antibody (RGAR003, Proteintech, 1:100) and CoraLite®647 conjugated goat anti-mouse secondary antibody (SA00014-10, Proteintech, 1:100).

Techniques: Expressing, Marker

Journal: Journal of Translational Autoimmunity

Article Title: TGF-β1 promotes collagen synthesis in systemic sclerosis via upregulating P4HA3

doi: 10.1016/j.jtauto.2025.100323

Figure Lengend Snippet: P4HA3 and TGF-β1 are increased in SSc skin tissue. (A) Representative immunohistochemistry images of P4HA3 staining in the skin from healthy controls (HC) and SSc. The numbers of P4HA3-positive cells were blindly counted in the skin of 4 HC and 12 SSc. Scale bars = 500 μm (left) or 50 μm (right). (B) Representative IF images of P4HA3 (green) and SFRP2 (red) staining merged with nuclear marker DAPI (blue) in the skin from HC and SSc. Following IF imaging, the same tissue sections were subjected to hematoxylin and eosin (H&E) staining. Scale bars = 200 μm (H&E) 20 μm (IF). (C) Representative immunohistochemistry images of TGF-β1 staining in the skin from HC and SSc. The numbers of TGF-β1-positive cells were blindly counted in the skin of 4 HC and 12 SSc. Scale bars = 500 μm (left) or 50 μm (right). Arrow represents positive cells. ∗∗ P < 0.01 by Mann–Whitney U test.

Article Snippet: Tissue slices were incubated overnight at 4 °C with rabbit anti-P4HA3 antibody (HPA007897, Sigma, 1:150) and mouse anti-SFRP2 antibody (66328-1-Ig, Proteintech, 1:100), followed by staining with CoraLite®555 conjugated goat anti-rabbit secondary antibody (RGAR003, Proteintech, 1:100) and CoraLite®647 conjugated goat anti-mouse secondary antibody (SA00014-10, Proteintech, 1:100).

Techniques: Immunohistochemistry, Staining, Marker, Imaging, MANN-WHITNEY